Phenotype Image Detail

Image

Caption

Reduction in brain size in Gpt2^{tm1a(KOMP)Wtsi}/Gpt2^{tm1a(KOMP)Wtsi} (Gpl2 MUT) mice. (A) Panels depict two representative brain pairs from littermate animals for each genotype at P21 (Top) and P22 (Bottom). Left panels show wild-type (WT) perfused brains. Right panels show mutant brains (MUT). Measurements were taken of the cortex (CX), the midbrain (MB), the cerebellum (CB), the left hemisphere (LH), and the right hemisphere (RH). Ruler demarcations reflect 1-mm scale. (B) Cortical area presented as a percentage of total brain area is shown for WT animals and for mutant animals. **P < 0.03. (C) Graphed is a comparison of area in square centimeters in four different brain regions: LH (**P < 0.0025), RH (**P < 0.0015), CX+MB+CB (*P < 0.0015), and MB+CB (n.s., P < 0.1). (D) Graphed is a comparison of length measurements between WT and mutant animals. Measurements are depicted in the brain image on the Right. We found significant differences in width across two hemispheres (L1, green line) (*P < 0.015), in the midline anterior-posterior distance (L2, red line) (**P < 0.0015), in the diagonal length of the left hemisphere (L3, blue line) (***P < 0.0002), and in the right hemisphere (L4, yellow line) (****P < 0.000001). n = 7 WT and 12 MUT animals for all brain size measurements. Data are presented as means SEMs. Statistical analysis was conducted using Student's t test. (E) Mutant hippocampal neurons show reduced synapses compared with neurons from WT littermates at 10 DIV, as visualized by SV2 staining (green). Neurons were immunostained with antibodies against SV2 and MAP2 (red). (Scale bar, 10 um.) (F) The graph depicts quantification of SV2 densities per 135-um² region of field of view in WT and MUT neurons. Data are presented as means SEMs. n = 4 WT animals and 3 MUT animals across 3 litters. ***P = 0.005.

Copyright

This image is from Ouyang Q, Proc Natl Acad Sci U S A 2016 Sep 20;113(38):E5598-607. Copyright 2016 National Academy of Sciences, U.S.A. J:238353

Associated Alleles

Symbol	Name
Gpt2^tm1a(KOMP)Wtsi	glutamic pyruvate transaminase (alanine aminotransferase) 2; targeted mutation 1a, Wellcome Trust Sanger Institute
Gpt2^tm1a(KOMP)Wtsi	glutamic pyruvate transaminase (alanine aminotransferase) 2; targeted mutation 1a, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Gpt2^tm1a(KOMP)Wtsi/Gpt2^tm1a(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N