Phenotype Image Detail

Image

Caption

Visualization of colocalization of lysosomes with autophagosomes. Lens epithelial cells from wild-type (WT) and Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) lenses were cultured and immunostained with anti-Lamp-II antibody (red) to visualize lysosomes followed by staining with LC3B/MAP1LC3B antibody conjugated with AlexaFluor 488 (green) and DAPI nuclear stain (blue) to label autophagosomes and nuclei respectively. Panels (A-D) show immunostaining of WT lens epithelial cells and (E-H) of epithelial cells from KO mice lenses. (Data is representative of 8-10 cells for each type). Scale bars: 20 um.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N