Phenotype Image Detail

Image

Caption

Nuclear degradation process is impaired in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} mice. A. H&E staining of 1-month old wild-type (WT), Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1⁺ (HET) and Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) mice lens sections. An arrow in KO lens image show an abnormally high number of nuclei compared to WT and HET lenses. B. DAPI-staining of 1-month old lens sections of WT, HET and KO mice. DAPI-staining shows an accumulation of nuclei and nuclear debris in the OFZ (organelle free zone) and in nuclear region of KO lenses (indicated by an arrow). The bar graph on the right shows the quantification of nuclei. Data represent mean S.D in n = 4 independent experiments. **, P<0.01 by student's t test. Scale bars: A: 100 um, B: 50 um.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N