Phenotype Image Detail

Image

Caption

Increased radial migration in the olfactory bulb (OB) of Plxnb2^tm1Matl/Plxnb2^tm1Matl (Plxnb2-/-) mice. A-D, Coronal sections of the OB of heterozygous (A, B) and homozygous mutant adult mice (C, D) 4 days after an injection of BrdU. In the heterozygotes, almost all the BrdU+ cells are found in the rostral migratory stream (RMS) (A, B, arrowheads) and only a few have entered the granule cell layer (GCL) (A, arrow). By contrast, in the homozygous mutant, BrdU+ cells are found in the RMS (arrowheads) but many have entered the granule cell (GC) layer and periglomerular cells (PG) layer (arrows). B and C are counterstained with Hoechst. E is a quantification of the distribution of BrdU+ cells inside and outside of the RMS. In the OB of Plxnb2 mutant, there is a significant increase in the number of BrdU+ cells found outside of the RMS. Error bars indicate SEM. F-I are sagittal sections of the OB of P7 heterozygotes (F, G) and homozygotes (H, I ) electroporated at P2 in the subventricular zone (SVZ) with a green fluorescent protein (GFP) plasmid. Whereas in heterozygotes only a few GFP+ cells have started to migrate into the GCL (arrows), in homozygous mutant mice, many GFP+ cells have already left the RMS. G and H are higher magnification pictures of the region indicated by an asterisk in F and I (Hoechst counterstaining). Scale bars: A, D, F, H, 600 um; B, C, G, I, 300 um.

Copyright

This image is from Saha B, J Neurosci 2012 Nov 21;32(47):16892-905, an open-access article distributed under the terms of the Creative Commons Attribution Non-Commericial License. J:190014

Associated Alleles

Symbol	Name
Plxnb2^tm1Matl	plexin B2; targeted mutation 1, Marc Tessier-Lavigne

Associated Genotypes

Allelic Composition	Genetic Background
Plxnb2^tm1Matl/Plxnb2^tm1Matl	involves: 129P2/OlaHsd * CD-1