Phenotype Image Detail

Image

Caption

DNA double strand breaks persist in Kash5^{tm1b(KOMP)Wtsi}/Kash5^{tm1b(KOMP)Wtsi} testes. (A) Low and (B) high magnification views of testis sections from wild-type (WT) and Kash5^{tm1b(KOMP)Wtsi}/Kash5^{tm1b(KOMP)Wtsi} (Kash5-null) animals, showing the distribution of DNA double strand breaks (DSBs) revealed by Gamma-H2AX labeling. In WT testis sections, a ring of Gamma-H2AX-positive nuclei can sometimes be seen at the periphery of seminiferous tubules. The DSBs are always resolved, with a concomitant decline in Gamma-H2AX levels, as spermatocytes mature and progress spatially toward the center of the tubule. Mutant testes display persistent Gamma-H2AX-positive cells that accumulate toward the center of the tubule. (D) Spermatocyte spreads immunostained with anti-SCP3 and anti-Rad51 antibodies. In spreads from WT animals only the occasional cell is positive for both proteins (top row). Distribution of SCP3 indicates that these are leptotene/zygotene cells. The bulk of cells positive for SCP3, typically in pachytene, have resolved the majority of the Rad51 foci (middle row). The preponderance of SCP3-positive mutant spermatocytes that never reach pachytene remain Rad51-positive (bottom row).

Copyright

This image is from Horn HF, J Cell Biol 2013 Sep 30;202(7):1023-39, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:201813

Associated Alleles

Symbol	Name
Kash5^tm1b(KOMP)Wtsi	KASH domain containing 5; targeted mutation 1b, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Kash5^tm1b(KOMP)Wtsi/Kash5^tm1b(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N