Phenotype Image Detail

Image

Caption

Abnormal response of Raf1^tm1.1Bgn/Raf1⁺ (L613V/+) mice to pressure overload. (B) Gross appearance of hearts and heart weight/BW ratio at 8 weeks after transverse aortic constriction (TAC) or sham surgery. Dashed outlines demonstrate markedly enlarged left atrium in Raf1^tm1.1Bgn/Raf1⁺ compared with wild-type (WT) mice. **P < 0.005, ***P < 0.0001, Bonferroni post-test when ANOVA was significant; ##P < 0.005, 1-tailed Student's t test. (C) Severe interstitial fibrosis in mutant hearts (PSR staining; original magnification, 100) at 8 weeks after TAC. Percent pixels staining positive with PSR for interstitial fibrosis was quantified using ImageJ. n = 14 (WT); 13 (L613V/+). ***P < 0.0001, Bonferroni post-test when ANOVA was significant; #P < 0.05, 2-tailed Student's t test. (D) Perivascular fibrosis in hearts (PSR staining; original magnification, 200) at 8 weeks after TAC. Similar results were obtained when Masson Trichrome stain was used to assess fibrosis.

Copyright

This image is from Wu X, J Clin Invest 2011 Mar 1;121(3):1009-25 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:172034

Associated Alleles

Symbol	Name
Raf1^tm1.1Bgn	v-raf-leukemia viral oncogene 1; targeted mutation 1.1, Benjamin G Neel

Associated Genotypes

Allelic Composition	Genetic Background
Raf1^tm1.1Bgn/Raf1^+	involves: 129S6/SvEvTac * C57BL/6NCr