Phenotype Image Detail

Image

Caption

Intestinal ablation of Neurog3 leads to an altered morphology of the small intestine but normal Paneth and goblet cell differentiation. Sections of adult wild-type and Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 and Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 (mutant) duodenum, jejunum, and ileum were examined for their overall appearance (A-D) and the presence of Paneth (E and F) and goblet cells (G and H). Images presented are from the jejunum of control (A, C, E, and G) and mutant (B, D, F, and H) animals and are also representative for the phenotype observed in the duodenum and ileum of mutant animals. (A and B) H&E staining clearly shows the frequent blunt or club-shaped appearance of the villi and the disorganization of the crypt compartment of mutant animals compared with control small intestine. (C and D) Immunofluorescence analyses with an antibody recognizing all laminins, showing the frequent detachment of the intestinal epithelium from the lamina propria in mutant small intestine. (E and F) Immunohistochemistry with an anti-lysozyme antibody demonstrates normal appearance and location of Paneth cells (arrows in E and F) in mutant small intestine. (G and H) Likewise, periodic acid-Schiff staining shows that intestinal ablation of Neurog3 does not alter the location or number of goblet cells (arrows in G and H) in mutant animals. The age of the animals analyzed is 10-12 weeks.

Copyright

This image is from Mellitzer G, J Clin Invest 2010 May 3;120(5):1708-21 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161480

Associated Alleles

Symbol	Name
Neurog3^tm1Fgu	neurogenin 3; targeted mutation 1, Francois Guillemot
Neurog3^tm3.1Ggr	neurogenin 3; targeted mutation 3.1, Gerard Gradwohl
Tg(Vil1-cre)20Syr	transgene insertion 20, Sylvie Robine

Associated Genotypes

Allelic Composition	Genetic Background
Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL
Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL