Phenotype Image Detail

Image

Caption

Degeneration of outer hair cells in the cochleas of Cldn9^nmf329/Cldn9^nmf329 mice after P8. (A-I) Organ of Corti preparations from wild-type (+/+) and mutant mice were stained with phalloidin-Alexa Fluor 488 to visualize actin-rich structures including the stereocilia. At P8, the bundles of stereocilia are present in all three rows of outer hair cells (OHCs) (arrowheads) and in the row of IHCs (arrow) in the cochleas of both wild-type (A) and mutant mice (B, C). At P14, the basal turn of the wild-type cochlea contains three intact rows of OHCs (D), whereas the corresponding region of the mutant cochlea lacks the majority of stereociliary bundles (E). Where the cell-cell junctions are in focus, large polygonal cells can be seen to replace the missing OHCs (center of panel E). At the apical turn of the same cochlea, a few stereociliary bundles are missing from the first row of OHCs (F). At P80, the control cochlea is undamaged (G), but the mutant cochlea contains only a small number of OHCs at the basal turn, and the majority of OHCs are replaced by non-ciliated cells (H). At the apical turn of the mutant cochlea (P80), several OHCs are replaced by large polygonal cells, especially in the first row (I). Scale bars: 10 um.

Copyright

This image is from Nakano Y, PLoS Genet 2009 Aug;5(8):e1000610, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152154

Associated Alleles

Symbol	Name
Cldn9^nmf329	claudin 9; neuroscience mutagenesis facility, 329

Associated Genotypes

Allelic Composition	Genetic Background
Cldn9^nmf329/Cldn9^nmf329	C57BL/6J-Cldn9^nmf329/J