Phenotype Image Detail

Image

Caption

Compromised skeletal development in n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0 (Trsp^fl/fl Col2a1-Cre) mice. Radiographs of representative control (left) and mutant (right) mice. Arrows within the magnified areas highlight the irregularly-shaped vertebral bodies and narrowed intervertebral disc spaces in the mutant spinal column. (B) Ventral and lateral micro-CT views of lumbar vertebrae from control (left) and mutant (right) mice showing delayed spinal column ossification and loss of intervertebral disc spaces. (C) Micro-CT scans of the dorsal aspect of control (left) and experimental (right) skulls show smaller overall head size, rounding of the cranium (with possible decreased frontal bone ossification), and nasal bone shortening in the mutant mouse. (D) Generalized shape images of representative skulls. Ten mutant mice were used to generate this phenotypic average for the mutant mice (red skull) and nine controls were used (grey skull); the overlay of the images is presented beneath. Note that skull sizes from the control and experimental groups have been normalized, thus, only differences in shape of the cranium are being revealed by the overlay technique. (E) Anterior micro-CT views of knee joints from control (left) and experimental (right) mice showing smaller overall size in the latter, yet with relatively wide growth plates being present in the mutant tibia and fibula. (F) Cortical bone thickness difference (p= 0.0005) between the genotypes at the mid-shaft of the femur measured by micro-CT (n= 5). Data are mean+/- SEM, and were from 3.5-4 week old mice.

Copyright

This image is from Downey CM, PLoS Genet 2009 Aug;5(8):e1000616, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152152

Associated Alleles

Symbol	Name
n-TUtca2^tm1Dhat	nuclear encoded tRNA selenocysteine 2 (anticodon TCA); targeted mutation 1, Dolph L Hatfield
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0	B6.Cg-n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr