Phenotype Image Detail

Image

Caption

NeuN staining for neuronal diameter measurements in Tsc1^tm1Djk/Tsc1^tm1Djk Gt(ROSA)26Sor^tm1Sho/Gt(ROSA)26Sor⁺ mice injected with an adenovirus expressing cre recombinase (AAV1-CBA-Cre injected). Immunostaining for NeuN (counterstained with haematoxylin) shows that neurons above the lateral ventricles in (A) AAV1-CBA-Cre injected Tsc1^tm1Djk/Tsc1^tm1Djk Gt(ROSA)26Sor^tm1Sho/Gt(ROSA)26Sor⁺ mice were considerably larger than those in controls in the same region: (B) AAV1-CBA-GFP injected and (C) uninjected mice. Magnification = 40X. (D) Periventricular neurons in the brains of AAV1-Cre and AAV1-GFP injected, as well as non-injected mice (P30) were stained for the neuronal marker, NeuN and the widest diameter of stained cell bodies was measured in >30 randomly selected cells from several fields with 3 animals per group. Neurons in the AAV1-CBA-Cre injected brains had an almost 2-fold increase in diameter, as compared to controls, shown as mean +/-SEM (*p<0.0001).

Copyright

This image is from Prabhakar S, PLoS One 2013;8(5):e64224, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:221022

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1Sho	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Stuart Orkin
Tsc1^tm1Djk	TSC complex subunit 1; targeted mutation 1, David J Kwiatkowski

Associated Genotypes

Allelic Composition	Genetic Background
Tsc1^tm1Djk/Tsc1^tm1Djk Gt(ROSA)26Sor^tm1Sho/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * 129S4/SvJaeSor