Phenotype Image Detail

Image

Caption

Histological analysis showing reduced re-epithelialisation, impaired collagen deposition and increased cellular infiltration in Hspb1^tm1.1Jdn/Hspb1^tm1.1Jdn (hspB1^del/del) relative to wild-type (hspB1^+/+) wounds. A, Masson's trichrome staining of wild-type and mutant d3 and B, d7 wounds; bar = 500 um. C, High power images of cellular infiltrate from boxed regions indicated in (A) are shown; bar = 100um. D, as for (C) but at higher magnification showing cells with multi-lobed nuclei characteristic of neutrophils; (bar = 20 um). E, Plot showing mean areas of incomplete collagen deposition in d7 wild-type and mutant wounds (n = 5); **P<0.01. F, Plot of mean distance +/- SEM (n = 3 mice per group) between re-epithelialisation margins (re-ep) in wild-type and mutant mice in d3 wounds; (*P<0.05 calculated by Student's t-test of 12 individual wounds per group from 3 experiments). 11-14 week age-matched female wild-type and mutant mice were used.

Copyright

This image is from Crowe J, PLoS One 2013;8(10):e77383, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209117

Associated Alleles

Symbol	Name
Hspb1^tm1.1Jdn	heat shock protein 1; targeted mutation 1.1, Jonathan Dean

Associated Genotypes

Allelic Composition	Genetic Background
Hspb1^tm1.1Jdn/Hspb1^tm1.1Jdn	B6.129S2(Cg)-Hspb1^tm1.1Jdn