Phenotype Image Detail

Image

Caption

Abnormal GABAergic differentiation in Gbx1^tm1Mrh/Gbx1^tm1Mrh (Gbx1-/-) mice. Expression of Gad67 in wild-type (WT) (A, C) and mutant (B, D) mice at E18.5 (n = 3). Higher magnification views (C, D; areas boxed in A, B) show the dorsal horn, in which cell countings were performed. Expression of Pax2 in WT (E) and mutant (F) mice at E18.5 (n = 3). Expression of Slc17a6 in WT (G) and mutant (H) mice at E18.5 (n = 3). (I) Countings (percentages of labelled vs total cells) revealed that the proportion of Gad67+ cells is diminished by 16% in mutant mice (50.89% 2.61 Gad67+ cells in WT; 34.85% 1.84 in mutant mice; Genotype F(1,4) = 223.85, ***p < 0.001, Sections F(2,8) = 2.22, NS, Genotype* Sections F(2,8) = 1.23, NS). Also, the proportion of Pax2+ cells is diminished by 14.7% in mutant mice (58.57% 4,03 Pax2+ cells in WT; 42.41% 5.96 in mutant mice; Genotype F(1, 4) = 449.36, ***p < 0.001, Sections F(2, 8) = 3.34, NS, Genotype* Sections F(2, 8) = 6.3, p < 0.05). In contrast, countings revealed that the proportion of Slc17a6+ cells is increased by 14.4% in mutant mice (50.96% 1.84 Slc17a6+ cells in WT; 65.16% 2.94 in mutant mice; Genotype F(1, 4) = 688.84, ***p < 0.001, Sections F(2, 8) = 1.004, NS, Genotype* Section F(2, 8) = 4.73, p<0.05). Scale bars: 100 um.

Copyright

This image is from Meziane H, PeerJ 2013;1():e142, an open-access article distributed under the terms of the Creative Commons Attribution License. J:201154

Associated Alleles

Symbol	Name
Gbx1^tm1Mrh	gastrulation brain homeobox 1; targeted mutation 1, Muriel Rhinn

Associated Genotypes

Allelic Composition	Genetic Background
Gbx1^tm1Mrh/Gbx1^tm1Mrh	involves: 129P2/OlaHsd * C57BL/6J