Gene Expression Image Detail

Reference

Figure

ID

J:182719 Mikedis MM, et al., Dev Biol. 2012 Mar 1;363(1):201-18

2

MGI:5619291

Image

Caption

Immunostaining (brown color) with hematoxylin counterstain (blue) on sections (sagittal, unless indicated, with anterior to the left and posterior to the right). (A1-3) 7-s stage (~8.5 dpc). The site of amniotic insertion at the embryonic/extraembryonic junction (black arrowheads in A1-2), the vessel of confluence in the allantois (red arrowhead outlined in black, A3). Red asterisk marks vessel of confluence. (B) 8-s stage (~8.75 dpc). The midregion of the allantois (box; higher magnification in inset). (C1-5) 6-s stage (~8.5 dpc), transverse profile with dorsal to the left and ventral to the right of each panel. This sequence begins at the hindgut opening and proceeds posteriorly toward the allantois. (C1) 18 um below the anterior-most segment of closed hindgut tube. Presumptive dorsal hindgut endoderm (red arrowhead outlined in black). (C2) Anterior-most segment of closed hindgut tube. (C3) More posteriorly (24 um posterior to the anterior-most segment of the closed hindgut tube, and 36 um anterior to the end of the hindgut tube). (C4) 12 um posterior to the end of the hindgut tube. (C5) 132 um and 144 um posterior to base of the allantois and the end of the hindgut, respectively. (D) 9-s stage (~8.75 dpc). (E1-3) 11-s stage (~9.0 dpc), transverse profile. The tail has turned, and the allantois has shifted its position to lie in the posterior ventral midline. These sections were taken at the level of the closed hindgut tube anterior (E1) and posterior (E2-3) to the allantoic insertion into the tailbud. (E3) Box; higher magnification in inset. Other abbreviations: ne, neurectoderm; and oa, omphalomesenteric artery. Scale bar in A1=27.3 um (A1-3), 35.7 um (B), 9.0 um (B inset), 37.8 um (C1-3), 26.1 um (C4), 25.1 um (C5), 42.3 um (D), 30.6 um (E1), 55.3 um (E2-3), and 8.3 um (E3 inset).

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2011.10.003 Dev Biol 363: 201-18, Mikedis MM; Downs KM, STELLA-positive subregions of the primitive streak contribute to posterior tissues of the mouse gastrula. Copyright 2012

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A1	MGI:5619305 (Dppa3)
A2	MGI:5619305 (Dppa3)
A3	MGI:5619305 (Dppa3)
B	MGI:5619305 (Dppa3)
C1	MGI:5619305 (Dppa3)
C2	MGI:5619305 (Dppa3)
C3	MGI:5619305 (Dppa3)
C4	MGI:5619305 (Dppa3)
C5	MGI:5619305 (Dppa3)
D	MGI:5619305 (Dppa3)
E1	MGI:5619305 (Dppa3)
E2	MGI:5619305 (Dppa3)
E3	MGI:5619305 (Dppa3)