Gene Expression Image Detail

Reference

Figure

ID

J:182719 Mikedis MM, et al., Dev Biol. 2012 Mar 1;363(1):201-18

1

MGI:5619289

Image

Caption

Immunostaining (brown color) with hematoxylin counterstain (blue) on sections (sagittal, unless indicated, with anterior to the left and posterior to the right). Arrowheads indicate staining. (A) 4-s stage. Allantois (al). (B) Minus antibody control. Arrowhead indicates loss of staining. Trophoblast giant cells (gc). (C-D) E12.5. Developing female (C) and male (D) gonads. (E) OB stage (~7.0 dpc). XPS (extraembryonic primitive streak, xps). (F) Late Bud (LB) stage (~7.5 dpc). A cluster of positive cells (outlined by red dotted line). IPS (intraembryonic primitive streak, ips). (higher magnification in inset). AX (allantois-associated extraembryonic visceral endoderm; ax) denoted by red arrowhead. (G) Late Headfold (LHF) stage (~8.0 dpc). Red dotted line outlines a cluster of positive cells. EVE (embryonic visceral endoderm). ACD, delineated by brackets, representing average length of 120 um. Positive cell in amniotic ectoderm denoted by black arrowhead and displayed at higher magnification in inset. (H1-3) 2-s stage (~8.25 dpc), transverse profile with ventral toward the top and dorsal toward the bottom of each panel. Sections represent: the IPS 18 um below allantois (H1), the base of the allantois at theembryonic/extraembryonic boundary (H2), and 18 um above the base of the allantois (H3). Asterisk denotes approximate center of the IPS or ACD in each section. (I-J) 3-s and 5-s stages (~8.25 dpc). ACD (black asterisk), hindgut (hg; J inset). Ventral cuboidal mesothelium (VCM), extraembryonic visceral endoderm (xve; red arrowhead). Amniotic ectoderm denoted by black arrowhead and displayed at higher magnification in inset (I). Red asterisk (J) marks vessel of confluence. Other abbreviations: ac, amniotic cavity; am, amnion; hf, headfolds; and x, exocoelomic cavity. Scale bar in A=200.0 um (A-B), 26.7 um (C-D), 24.8 um (E-G, I, J), 8.3 um (F inset), 8.4 um (G inset), 34.2 um (H1-3), 7.3 um (I inset), and 56.5 um (J inset).

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2011.10.003 Dev Biol 363: 201-18, Mikedis MM; Downs KM, STELLA-positive subregions of the primitive streak contribute to posterior tissues of the mouse gastrula. Copyright 2012

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:5619305 (Dppa3)
C	MGI:5619305 (Dppa3)
D	MGI:5619305 (Dppa3)
E	MGI:5619305 (Dppa3)
F	MGI:5619305 (Dppa3)
G	MGI:5619305 (Dppa3)
H1	MGI:5619305 (Dppa3)
H2	MGI:5619305 (Dppa3)
H3	MGI:5619305 (Dppa3)
I	MGI:5619305 (Dppa3)
J	MGI:5619305 (Dppa3)
J inset	MGI:5619305 (Dppa3)
G inset	MGI:5619305 (Dppa3)