Phenotype Image Detail

Image

Caption

Stains show thickened diaphragmatic tissue caused by an increase in cell number in Kif7^dda/Kif7^dda (Kif7^dd/dd) embryos. (E and F) H&E-stained sagittal sections of E14.5 wild-type (WT) and mutant diaphragms show thickened diaphragmatic tissue in mutant embryos (arrowhead). (G and H) Hoechst nuclear stain of E13.5 embryos shows that thickened diaphragmatic tissue is because of increased cell number in mutant embryos (diaphragms outlined with dotted lines). (I and J) Immature diaphragms from E13.0 WT and mutant embryos show an early bilateral accumulation of extra diaphragmatic tissue in mutant embryos (arrowhead). (K and L) Wt1 (green) and Hoechst (blue) immunofluorescent staining of E13.5 WT and mutant diaphragms show an accumulation of Wt1+ cells in the mutant diaphragm. (M and N) Whole-mount in situ hybridization shows Kif7 expression in the posterior immature diaphragm (inside dotted lines) at (M) E11.5 and (N) E12.5. (O and P) Section in situ hybridization of Kif7 in (O) E11.5 and (P) E12.5 immature diaphragms (arrowheads point to diaphragm expression). (Q-T) Whole-mount in situ hybridization in E12.5 WT and mutant diaphragms. Note that there is increased Gli1 expression in the posterior mutant diaphragm (region delineated by dotted line), whereas there is no Ptch1 expression in either the WT or mutant.

Copyright

This image is from Coles GL, Proc Natl Acad Sci U S A 2013 May 21;110(21):E1898-905. Copyright 2013 National Academy of Sciences, U.S.A. J:197449

Associated Alleles

Symbol	Name
Kif7^dda	kinesin family member 7; disorganized diaphragm allele

Associated Genotypes

Allelic Composition	Genetic Background
Kif7^dda/Kif7^dda	FVB.A-Kif7^dda