Phenotype Image Detail

Image

Caption

Tumorigenic anomalies in tamoxifen-treated Mob1a^tm1.1Asuz/Mob1a^tm1.1Asuz Tg(KRT14-cre/ERT)20Efu/0 Mob1b^{Gt(CC0690)Wtsi}/Mob1b^{Gt(CC0690)Wtsi} (kDKO) keratinocytes. (A) Anti-Ki67 immunostaining of interfollicular epidermis (IFE) (left) and hair follicle (HF) (middle) of control and kDKO(P1) mice at P13. Scale bars: 50 um. Quantitation of Ki67+ cells (right); *P < 0.01. (B) Histology (left) and quantitation (right) of TUNEL-stained cells in epidermis from control and kDKO(P1) mice at P16. Scale bar: 50 um; *P < 0.01. (C) Keratinocytes from control and kDKO(P1) mice at P4 were cultured for the indicated number of days, and total cell numbers were counted. kDKO(P1) keratinocytes achieved higher saturation plating density; *P < 0.01. (D) Left: H&E-stained epidermal basal layer of control and kDKO(P1) mice at P19. Scale bar: 20 um. Right: quantitation (cell number/50 um BM); n = 5/group; *P < 0.05. (E-G) Immunostaining to detect gamma-Tubulin (green) and alpha-Tubulin (red) in control and kDKO(P1) keratinocytes. DAPI, nuclei. Mutant keratinocytes showed excess centrosomes (E), multi-polar spindles (F), and micronuclei (G). Scale bars: 20 um; *P < 0.05.

Copyright

This image is from Nishio M, J Clin Invest 2012 Dec 3;122(12):4505-18 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:193981

Associated Alleles

Symbol	Name
Mob1a^tm1.1Asuz	MOB kinase activator 1A; targeted mutation 1.1, Akira Suzuki
Mob1b^Gt(CC0690)Wtsi	MOB kinase activator 1B; gene trap CC0690, Wellcome Trust Sanger Institute
Tg(KRT14-cre/ERT)20Efu	transgene insertion 20, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Mob1a^tm1.1Asuz/Mob1a^tm1.1Asuz Mob1b^Gt(CC0690)Wtsi/Mob1b^Gt(CC0690)Wtsi Tg(KRT14-cre/ERT)20Efu/0	involves: 129P2/OlaHsd * C57BL/6 * CD-1