Phenotype Image Detail

Image

Caption

Scanning electron micrographs of P2-5 vestibular epithelia. (A-C and J) wild-type (Myo7a+/+), (D-F and K) Myo7a^ewaso/Myo7a^ewaso (Myo7a^I487N/I487N), and (G-I) Myo7a^dmbo2/Myo7a^dmbo2 (Myo7a^F947I/D947I) mice. A clear line of polarity reversal can be identified in wild-type saccular maculae (A; dashed line), and normal hair bundle morphology is apparent, with a staircase arrangement of stereocilia and kinocilium located with the tallest stereocilia (B and C). Hair cell bundles are affected in Myo7a^ewaso/Myo7a^ewaso saccules, and no clear zone of polarity is evident (D). These bundles do contain stereocilia of various lengths, however are generally disorientated and unstructured (E and F). A zone of polarity can be identified in Myo7a^dmbo2/Myo7a^dmbo2 saccules (G), however hair bundles do appear to have a mild phenotype, with bundles missing some of the tallest (H) and/or shortest stereocilia (I). Scale bar; 10 uM (A, D and G), 2 uM (B, C, E, F, H and I).

Copyright

This image is from Miller KA, PLoS One 2012;7(12):e51284, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195666

Associated Alleles

Symbol	Name
Myo7a^dmbo2	myosin VIIA; dumbo2
Myo7a^ewaso	myosin VIIA; ewaso

Associated Genotypes

Allelic Composition	Genetic Background
Myo7a^ewaso/Myo7a^ewaso	involves: C57BL/6J
Myo7a^dmbo2/Myo7a^dmbo2	involves: C57BL/6J