Phenotype Image Detail

Image

Caption

Abnormal ultrastructural features in Trim50^tm1Hta/Trim50^tm1Hta (KO) parietal cells. A, parietal cells in the resting and stimulated phases were examined by electron microscopy. In the resting phase, no difference was observed between the genotypes (left column). Note the irregular formation of intracellular canaliculi (IC) and excessive multilamellar membrane complexes (arrowhead) in mutant parietal cells during the stimulated phase (middle column). High magnification views clarify the differences between the firm cylindrical microvilli with central actin filaments (arrows) in wild-type and the swollen microvilli leading to pleomorphic canaliculi in mutant parietal cells (right column). The scale bars indicate 1 um (left and middle columns) or 0.2 um (right column). B, excessive multilamellar membrane complexes in mutant parietal cells. Electron microscopic immunolabeling shows specific labeling for H⁺/K⁺-ATPase on the multilamellar membrane complexes in stimulated mutant parietal cells. The scale bar indicates 0.5um. C, quantitative evaluation of the multilamellar membrane complexes in wild-type and mutant cells. Parietal cells (n = 30 in each genotype) were randomly selected, and the multilamellar membrane complexes (minor axis > 0.5 um, with triple or more lamellar membranes) were evaluated in electron micrographs using the freeware program Image J (version 1.45p). The data represent the mean +/- S.E., and statistical differences are indicated by asterisks (*, p<0.05 and **, P<0.01 in t test).

Copyright

This image is from Nishi M, J Biol Chem 2012 Sep 28;287(40):33523-32 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:191586

Associated Alleles

Symbol	Name
Trim50^tm1Hta	tripartite motif-containing 50; targeted mutation 1, Hiroshi Takeshima

Associated Genotypes

Allelic Composition	Genetic Background
Trim50^tm1Hta/Trim50^tm1Hta	involves: 129S4/SvJae * C57BL/6J