Phenotype Image Detail

Image

Caption

Dedifferentiation in the nasal septum of Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0 (Col2a1-cre;Apc^15lox/15lox) embryos at E16.5. (A, E) Hematoxylin/eosin staining, (B, F, H) immunostaining for beta-catenin combined with Alcian blue (AB) staining, and (C-D, G, I) gene expression analysis by in situ hybridization with indicated probes for chondrogenic differentiation on consecutive transversal sections of hte developing nasal septum of a mutant embryo and control littermate at E16.5. (F', G', H', I') High magnification pictures of the boxed regions in F, G, H, and I, respectively. Mesenchymal-like beta-catenin-positive cells (arrow in F, H) were present between crumbled cartilage islands. Within these cartilage islands, although displaying chondrocytic morphology and an Alcian blue stained matrix, most of the beta-catenin-positive cells did not express Sox9 (arrowheads in G') or Col2a1 (arrows in I'). Scale bars: 100 um in A, E, F, H; 5 um in F', H'.

Copyright

This image is from Miclea RL, BMC Dev Biol 2009;9():26, an open-access article, licensee BioMed Central Ltd. J:149225

Associated Alleles

Symbol	Name
Apc^tm1Rsmi	APC, WNT signaling pathway regulator; targeted mutation 1, Ron Smits
Tg(Col2a1-cre)1Rsjo	transgene insertion 1, Randall S Johnson

Associated Genotypes

Allelic Composition	Genetic Background
Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0	involves: 129P2/OlaHsd * FVB/N