Phenotype Image Detail

Image

Caption

Antxr2 is required for murine parturition. (A) Genotyping of offspring from Antxr2^tm1.1Jkit/Antxr2⁺ (Antxr2+/-) intercrosses revealed that Antxr2^tm1.1Jkit/Antxr2^tm1.1Jkit (Antxr2-/-) mice are viable. (B) RT-PCR analysis of Antxr2 transcript expression in wild-type (Antxr2+/+) and homozygous mutant MEFs. (C) Fertility analysis of 6-week-old female mice revealed that 100% of homozyous mutant mice are unable to give birth. (D) Left panel - Analysis of uterine tissue isolated on GD18.5 showed that homozygous mutant uterine horns lack muscle striations (black arrows in right panel) and exhibit poor uterine tone (asterisk). Right panel- blow ups of boxed areas in left panel to highlight lack of striations in homozygous mutant uterine horns. (E) H&E staining and immunofluorescence for the smooth muscle cell marker, alpha-SMA, demonstrated that the circular and longitudinal myometrial cell layers are disrupted in GD18.5 homozygous mutant uterine tissue (Ep, endometrial epithelium; LM, longitudinal myometrium; CM, circular myometrium). Arrows point to remaining bundles of myometrial cells in homozygous mutant uterus. Immunostaining for Antxr2 revealed that the protein is expressed on myometrial cells in wild-type tissue (brown stain). Asterisks indicate bundles of myometrial cells that are negative for Antxr2 expression in homozygous mutant tissue. Masson's Trichrome staining demonstrated high collagen content (blue color) in area of homozygous mutant uterus where myometrial cell layers are usually present. (F) Masson's Trichrome staining of GD18.5 cervical tissue revealed a dense collagen network (blue color) in homozygous mutant cervices. Scale bars, 150 um.

Copyright

This image is from Reeves CV, PLoS One 2012;7(4):e34862, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187210

Associated Alleles

Symbol	Name
Antxr2^tm1.1Jkit	anthrax toxin receptor 2; targeted mutation 1.1, Jan K Kitajewski

Associated Genotypes

Allelic Composition	Genetic Background
Antxr2^tm1.1Jkit/Antxr2^tm1.1Jkit	involves: 129 * C57BL/6