Phenotype Image Detail

Image

Caption

Whole-mount light microscopy of Tgm3^tm1Sjo/Tgm3^tm1Sjo (A) and wild-type (B) hair (scale bar 10 um). Inserts show higher magnification of lower regions of the hairs. Hair incubated overnight at 65 degrees C with agitation in 2% SDS and 20 mM dithiothreitol (C-F) mutant (C, E) and control hairs (D, F). Arrowheads indicate regions lacking or retaining the cuticle cells in the mutant and control hairs, respectively. Scanning electron microscopy of mutant (G, I) and control (H, J) hair shafts shows severe distortion of the hair, in particular the cuticle, in the absence of Tgm3. In contrast, the roots of the mutant hairs (K) appear similar to the wild-type ones (L) (scale bar 20 um). Each of the four main pelage hair types was present in the mutant animals (M).

Copyright

This image is from John S, PLoS One 2012;7(4):e34252, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187109

Associated Alleles

Symbol	Name
Tgm3^tm1Sjo	transglutaminase 3, E polypeptide; targeted mutation 1, Susan John

Associated Genotypes

Allelic Composition	Genetic Background
Tgm3^tm1Sjo/Tgm3^tm1Sjo	involves: 129P2/OlaHsd * C57BL/6