Phenotype Image Detail

Image

Caption

Barrier function of the skin in Tgm3^tm1Sjo/Tgm3^tm1Sjo (TGM3-/-) mice. Transmission electron microscopy of skin from mutant (A, C and E) and wild-type (B, D and F) 4 week old animals. Cornified cell envelopes (closed arrowheads in A and B) and keratohyalin granules (*) were visible in mice of both genotypes and in some sections. The latter could be seen coalescing with the forming cornified envelope of granular layer keratinocytes (open arrowheads in C and D). The cells of the stratum corneum in both wild-type and mutant animals consisted of a defined cell envelope surrounding compact, electron-dense cytoplasm containing condensed tonofibrils. Lucifer yellow failed to penetrate through the cornified envelope in either the newborn mutant (G) or wild-type skin (H). (scale bars B 0.5 um, D 7 um, F and H 50 um). At birth there was no retention of toluidine blue dye in the skin of either mutant or wild-type neonates (I). Dye was retained in the basal region of the whiskers in mutant (L) neonates, a finding not seen in wild-type mice (K). While formation of the epithelial barrier had occurred in control mice at E17.5, toluidine blue penetrated the skin of mutant litter mates (J). Sonication of corneocytes isolated from skin biopsy punches for various times revealed that mutant corneocytes were more susceptible to lysis (M). The number shown is that of intact corneocytes remaining as a percentage of those initially isolated (n = 4).

Copyright

This image is from John S, PLoS One 2012;7(4):e34252, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187109

Associated Alleles

Symbol	Name
Tgm3^tm1Sjo	transglutaminase 3, E polypeptide; targeted mutation 1, Susan John

Associated Genotypes

Allelic Composition	Genetic Background
Tgm3^tm1Sjo/Tgm3^tm1Sjo	involves: 129P2/OlaHsd * C57BL/6