Phenotype Image Detail

Image

Caption

Impairment of angiogenesis by endothelial-specific deletion of Tmem100. (E) Endothelial-specific Tmem100 knockout embryos, Tmem100^tm1.1Ysai/Tmem100^tm2.1Ysai Tg(Tek-cre)1Ywa/0 (Tmem100^fl/ko;Tek-Cre+), show pericardial effusion (arrow) and severe growth retardation at E10.5. There are no major vitelline vessels in endothelial-specific Tmem100 knockout yolk sac. (F) Impairment of cardiovascular morphogenesis is observed in endothelial cell-specific Tmem100 knockout mice. As in global Tmem100 null mice, paired dorsal aortas (da) are highly dilatated or narrow in Tmem100^tm1.1Ysai/Tmem100^tm2.1Ysai Tg(Tek-cre)1Ywa/0 embryos. Trabeculae of ventricular (v) myocardium are not well formed in Tmem100^tm1.1Ysai/Tmem100^tm2.1Ysai Tg(Tek-cre)1Ywa/0 embryos. Yolk sacs of endothelial-specific Tmem100 knockout mice show large lacunae by a detachment of endodermal (en) and mesodermal (me) layers. H&E staining is shown. (Scale bars: E, 200 um; F, a-d, 50 um; and F, e and f, 20 um.)

Copyright

This image is from Somekawa S, Proc Natl Acad Sci U S A 2012 Jul 24;109(30):12064-9. Copyright 2012 National Academy of Sciences, U.S.A. J:186473

Associated Alleles

Symbol	Name
Tg(Tek-cre)1Ywa	transgene insertion 1, Masashi Yanagisawa
Tmem100^tm1.1Ysai	transmembrane protein 100; targeted mutation 1.1, Yoshihiko Saito
Tmem100^tm2.1Ysai	transmembrane protein 100; targeted mutation 2.1, Yoshihiko Saito

Associated Genotypes

Allelic Composition	Genetic Background
Tmem100^tm1.1Ysai/Tmem100^tm2.1Ysai Tg(Tek-cre)1Ywa/0	involves: C57BL/6 * SJL