Phenotype Image Detail

Image

Caption

Impairment of vascular remodeling and arterial endothelium differentiation in Tmem100^tm1.1Ysai/Tmem100^tm1.1Ysai (Tmem100 KO) embryos. (A) Impaired vascular remodeling is visualized by whole-mount Pecam1 immunostaining in the Tmem100 KO embryos (b, d, and f) and yolk sac (h). Arrows indicate intersomitic vessels. Arrowheads indicate highly organized vascular branches in the wild-type (WT) yolk sac. a-f are shown with nuclear DAPI stain. (B) Abnormal vascular connections (arrow) between dorsal aorta and cardinal veins are observed in null embryos. Shown is microangiography by India ink injection and H&E staining. (C and D) Expression of arterial endothelium marker genes Efnb2 and Gja5/Cx40 diminishes in dorsal aorta (arrowheads) of Tmem100 KO embryos at E8.5 (C) and E9.5 (D), whereas maintenance of Cdh5 expression indicates existence of endothelial layers. In situ hybridization is shown. (E) Flk1 and Pecam1 expression is preserved in dorsal aorta of Tmem100 KO embryo (b and d) and yolk sac vasculature (f). Immunohistochemistry with nuclear DAPI stain is shown. (F) Expression of Sm22/Tagln mRNA and SM22 protein in the mural layers of dorsal aorta is significantly reduced in Tmem100 KO embryos (arrowheads). In situ hybridization (a and b) and immunohistochemistry with nuclear DAPI stain (c and d) are shown. Insets in D and F show magnified views of dorsal aorta. (Scale bars: A, C, D, and E, a-d, 50 um; B, a-d, 200 um; and B, e and f, E, e and f, and F, 20 um).

Copyright

This image is from Somekawa S, Proc Natl Acad Sci U S A 2012 Jul 24;109(30):12064-9. Copyright 2012 National Academy of Sciences, U.S.A. J:186473

Associated Alleles

Symbol	Name
Tmem100^tm1.1Ysai	transmembrane protein 100; targeted mutation 1.1, Yoshihiko Saito

Associated Genotypes

Allelic Composition	Genetic Background
Tmem100^tm1.1Ysai/Tmem100^tm1.1Ysai	C57BL/6-Tmem100^tm1.1Ysai