Phenotype Image Detail

Image

Caption

Histological and histomorphometric analysis of wild-type (+/+), Cd68^tm1Xfen/Cd68⁺ (-/+), and Cd68^tm1Xfen/Cd68^tm1Xfen (-/-) mice. (A) Representative formalin-fixed, paraffin-embedded histological sections from each genotyped stained for TRAP activity and counterstained with hematoxylin at 40X magnification. (B) 400X magnification of area defined in (A). (C) Quantification of number of osteoclasts per bone surface (N, OC/BS). There was no significant difference in N. OC/BS between genotypes. (D) Representative 70% ethanol-fixed, plastic-embedded histological sections from each genotype stained with Goldner's trichrome at 40X magnification. (E) 400X magnification of area defined in (D). (F) Histomorphometric analysis of trichrome-stained sections. There was no significant difference between genotypes in numbers of osteoblasts (N. OB/BS) per bone surface. There was a significant increase in bone volume per total volume (BV/TV) in Cd68^tm1Xfen/Cd68^tm1Xfen mice compared to wild-type animals and trabecular number (N. Tb.) in homozygous mutant mice compared to wild-type and heterozygous mutant animals. (G) Quantification of mineral apposition rate (MAR) via analysis of calcein double labeling. The MAR was significantly higher in homozygous mutant mice compared to both wild-type and heterozygous mutant animals, and MAR was significantly higher in heterozygous mice compared to wild-type animals. *, p<.05; **, p<.01. Data presented are means + standard deviation.

Copyright

This image is from Ashley JW, PLoS One 2011;6(10):e25838, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:178120

Associated Alleles

Symbol	Name
Cd68^tm1Xfen	CD68 antigen; targeted mutation 1, Xu Feng

Associated Genotypes

Allelic Composition	Genetic Background
Cd68^tm1Xfen/Cd68^tm1Xfen	C57BL/6J-Cd68^tm1Xfen
Cd68^tm1Xfen/Cd68^+	C57BL/6J-Cd68^tm1Xfen