Phenotype Image Detail

Image

Caption

A, H&E-stained mouse brain showing a representative section that was stained in experiments detailed below. The box highlights the image magnified in panel B. Ob, olfactory bulb; Hyp, hypothalamus; Cor, cortex; Hip, hippocampus; Cb, cerebellum; PM, pons-medulla. Scale bar, 240 um. B, shown is a magnified image of a H&E section of mouse cerebellum. The box indicates a representative area of cerebellum stained for all subsequent analyses of all genotypes. Scale bar, 60 um. Immunohistochemical staining was performed on the Purkinje cell layer of serially sectioned wild-type (WT) (C and F), Sparcl1^tm1Pmc/Sparcl1^tm1Pmc (hevin-null) (D and G), and Adamts4^tm1Dqp/Adamts4^tm1Dqp (ADAMTS4-null) (E and H) mouse brains. Sections were stained with anti-calbindin (green). Altered morphology of Purkinje cell dendritic processes (arrows) is apparent in Sparcl1^tm1Pmc/Sparcl1^tm1Pmc (hevin-null) and Adamts4^tm1Dqp/Adamts4^tm1Dqp (ADAMTS4-null) tissue. Images are representative of staining patterns seen in three independent experiments. PC, Purkinje cell. Scale bar, 1um.

Copyright

This image is from Weaver MS, J Biol Chem 2010 Feb 19;285(8):5868-77 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:159752

Associated Alleles

Symbol	Name
Adamts4^tm1Dqp	ADAM metallopeptidase with thrombospondin type 1 motif 4; targeted mutation 1, Duanqing Pei
Sparcl1^tm1Pmc	SPARC-like 1; targeted mutation 1, Peter J McKinnon

Associated Genotypes

Allelic Composition	Genetic Background
Sparcl1^tm1Pmc/Sparcl1^tm1Pmc	involves: 129S1/Sv
Adamts4^tm1Dqp/Adamts4^tm1Dqp	involves: 129X1/SvJ * C57BL/6J