Phenotype Image Detail

Image

Caption

Histological and ultrastructural skin analysis of wild-type (+/+), Plec^tm7.1Gwi/Plec⁺ (Ogna/+) and Plec^tm7.1Gwi/Plec^tm7.1Gwi (Ogna/Ogna) mice. (A) Epoxy resin-embedded and toluidine blue-stained skin sections from 1-day-old mice. Asterisks indicate trauma-induced blister formation between stratum basale (sb) and dermis (d), not observed in wild-type (+/+) mice. sc, stratum corneum. (B) Transmission electron microscopy of skin lesions. Arrowheads indicate intact hemidesmosomes (HDs) with attached keratin filament bundles (arrows) aligned along the basal cell membrane of basal keratinocytes in wild-type-skin, and remnants of HDs lacking keratin filaments at blister (*) floors in mutant skin. (C, D) High magnification electron micrographs of intact skin sections of newborn (C) and adult (D) mice. (E) Outlines of HDs (grey bars) distributed along the basal cell membrane of basal keratinocytes (black lines) in panels D. Note reduced numbers and smaller sizes of HDs (arrowheads in C and D) in mutant, compared to wild-type skin; also, few HDs in mutant skin have attached keratin filament bundles, whereas thick bundles of filaments attached to HDs are dominant in wild-type skin (arrows in C and D). d and e (B-E), dermis and epidermis, respectively. Bars, 20 um (A); 500 nm (B-D).

Copyright

This image is from Walko G, PLoS Genet 2011 Dec;7(12):e1002396, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:179812

Associated Alleles

Symbol	Name
Plec^tm7.1Gwi	plectin; targeted mutation 7.1, Gerhard Wiche

Associated Genotypes

Allelic Composition	Genetic Background
Plec^tm7.1Gwi/Plec^tm7.1Gwi	involves: 129P2/OlaHsd * C57BL/6
Plec^tm7.1Gwi/Plec^+	involves: 129P2/OlaHsd * C57BL/6