Phenotype Image Detail

Image

Caption

A, leakage of Evans Blue in P7 Angptl4^tm1Vlcg/Angptl4⁺ (angptl4^+/LacZ) and Angptl4^tm1Vlcg/Angptl4^tm1Vlcg (angptl4^LacZ) retinas. Values are expressed as % of the control (Angptl4^tm1Vlcg/Angptl4⁺) value, (Angptl4^tm1Vlcg/Angptl4⁺, n=4; Angptl4^tm1Vlcg/Angptl4^tm1Vlcg, n = 5). B and G, confocal images of whole mount P7 Angptl4^tm1Vlcg/Angptl4⁺ and Angptl4^tm1Vlcg/Angptl4^tm1Vlcg retinas stained with isolectin B4 (IB4, blue) and ZO-1 (TJP1, red). Prior to sacrifice, fluorescent 100 nm microspheres were injected intravenously and flushed from circulation 15 min afterwards. B and C, deconvolution and three-dimensional reconstruction of the boxed region in E. Extravasated microspheres are trapped around tight junctions with a large part outside of the vessel (ZO-1, red). Field dimensions are 200 x 220 x 7 um. E and G, extravasated microspheres (green) were only observed in homozygous mutant retinas. Scale bar = 100 um. n = 6 per group. p < 0.05.

Copyright

This image is from Perdiguero EG, J Biol Chem 2011 Oct 21;286(42):36841-51 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:177790

Associated Alleles

Symbol	Name
Angptl4^tm1Vlcg	angiopoietin-like 4; targeted mutation 1, Velocigene

Associated Genotypes

Allelic Composition	Genetic Background
Angptl4^tm1Vlcg/Angptl4^tm1Vlcg	Not Specified