Phenotype Image Detail

Image

Caption

(A) Representative light microscopic pictures of transverse semithin sections from Sirt2^tm1.1Auw/Sirt2^tm1.1Auw (Sirt2fl/fl control) and Sirt2^tm1.1Auw/Sirt2^tm1.1Auw Tg(Mpz-cre)26Mes/0 (Sirt2-SCKO) sciatic nerves at the indicated postnatal ages. Note hypomyelination in Sirt2-SCKO mice with reduced number of myelinated fibers and thinner myelin sheaths at P1, P3, and P5, but no difference in nerve structure at later ages. (Scale bar: 10 um.) (B) Representative electron microscopy from P1 transverse sciatic nerve sections showing 1:1 relationship formation between axons (arrows) and individual Schwann cells (asterisks) in Sirt2-SCKO mice similar to that in control preparations. However, there is strikingly reduced compact myelin in Sirt2-SCKO mice compared with controls. Note higher density of SC-axon units in the Sirt2-SCKO preparation, which is typically observed in hypomyelination models. (Scale bar: 2 um.) (C) Quantification of DAPI-positive Schwann cell nuclei in transverse sciatic nerve sections from control and Sirt2-SCKO mice at P1, P3, and P5 (n = 3-5 mice for each group tested).

Copyright

This image is from Beirowski B, Proc Natl Acad Sci U S A 2011 Oct 25;108(43):E952-61. Copyright 2011 National Academy of Sciences, U.S.A. J:177468

Associated Alleles

Symbol	Name
Sirt2^tm1.1Auw	sirtuin 2; targeted mutation 1.1, Johan Auwerx
Tg(Mpz-cre)26Mes	transgene insertion 26, Albee Messing

Associated Genotypes

Allelic Composition	Genetic Background
Sirt2^tm1.1Auw/Sirt2^tm1.1Auw Tg(Mpz-cre)26Mes/0	involves: 129 * C57BL/6J * FVB/N