Phenotype Image Detail

Image

Caption

Mtss1^{Gt(CSC156)Byg}/Mtss1^{Gt(CSC156)Byg} (KO) MEFs were impaired in spreading during cell attachment. (A) Cells were trypsinized, plated on fibronectin-coated coverslips in a serum-containing medium, fixed at 30 min and 2 h and inspected by phase-contrast microscopy. An enlarged area in each 30-min image was shown in inset. (B) The number of cells with apparent cytoplasm extensions was countered at different times after plating. The data represents mean +/- SEM (n=3). The p value (Anova test) refers to the difference between Mtss1^{Gt(CSC156)Byg}/Mtss1^{Gt(CSC156)Byg} (KO) and wild-type (WT) cells during the course of attachment. (C) Cells at 2 h after plating were fixed, stained with phalloidin and scanned with confocal microscope with each optical section of 0.30 um in thickness. The images corresponding to different sections were compiled by Zeiss LSM Image browser.

Copyright

This image is from Yu D, PLoS One 2011;6(6):e20845, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:174444

Associated Alleles

Symbol	Name
Mtss1^Gt(CSC156)Byg	MTSS I-BAR domain containing 1; gene trap CSC156, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Mtss1^Gt(CSC156)Byg/Mtss1^Gt(CSC156)Byg	B6.129P2-Mtss1^Gt(CSC156)Byg