Phenotype Image Detail

Image

Caption

Tg(BEST1-HTRA1)#Ybf/0 (hHTRA1⁺) mice with polypoidal choroidal vasculopathy (PCV+) at 11 months of age exhibit degradation of the choroidal vessels. (A) H&E staining of retinal sections of an Tg(BEST1-HTRA1)#Ybf/0 mouse and a wild-type (WT) littermate. Large numbers of red blood cells are accumulated in the cavity between retinal pigment epithelium (RPE) and choroid (yellow arrows). There were nine pools of blood cells in four eyes of mutants with polypoidal choroidal vasculopathy (hHTRA1+ -PCV+) and no blood-cell accumulation in four eyes of WT littermates (one eye per mouse). Pools of blood cells were counted from one retinal/RPE/choroid section per eye. Black arrows point to RPE hypopigmentation. OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GC, ganglion cell layer. (Scale bars, 20 um.) (B) Comparison of choroid architecture between mutant and WT mice. Dilated, thin-wall vessels in mutant mice are indicated (red arrows). There were 23 thin-wall vessels in mutant PCV+ mice and only 2 in WT. Thirty-one choroidal regions from three mutant PCV+ mice (five eyes) and 30 choroidal regions from three WT (five eyes) were compared (0.75 +/- 0.14 per region in PCV+ vs. 0.07 +/- 0.05 in WT, P < 0.001). Thin plastic sections (200 nm) were stained with rabbit anti-L-aspartate IgG. (Scale bars, 50 um.) (C) Ultrastructural analysis showed severe degeneration of the elastic laminae (internal and external) and the tunica media of a choroid artery in mutant PCV+ mice. Lower panels show magnified views of the artery wall of a WT and a PCV+ mouse in the Upper panels (red boxes). [Scale bars, 2 um (Upper) and 1 um (Lower).]

Copyright

This image is from Jones A, Proc Natl Acad Sci U S A 2011 Aug 30;108(35):14578-83. Copyright 2011 National Academy of Sciences, U.S.A. J:175227

Associated Alleles

Symbol	Name
Tg(BEST1-HTRA1)#Ybf	transgene insertion, Yingbin Fu

Associated Genotypes

Allelic Composition	Genetic Background
Tg(BEST1-HTRA1)#Ybf/0	involves: C57BL/6J