Phenotype Image Detail

Image

Caption

Both male and female Mirc5^tm1Jae/Mirc5^tm1Jae (mir-290-295-/-) embryonic gonads show reduced germ cell numbers as early as E11.5. (A-D) Mouse vasa homolog (MVH) immunostaining of sections of E13.5 ovaries (A and B) and testes (C and D) from wild-type (WT) (A and C) and Mirc5^tm1Jae/Mirc5^tm1Jae (B and D) embryos. (Scale bars, 50 uM.) (E) Germ cell density (germ cells/area of gonadal section for ovaries and germ cells/testis cord area for testes) as determined by MVH staining. Each bar represents a single embryo with black bars indicating male embryos and white bars, female embryos. (F-I) MVH immunostaining of E11.5 embryo sections from WT (F and H) and mutant (G and I) embryos. (Scale bars, 50 uM.) White arrows point to germ cells in G and I. The additional green cells in I are blood cells. (J) Total germ cell numbers in E11.5 gonads as determined by serial sectioning and MVH staining. Each bar represents a single embryo with black bars indicating male embryos and white bars, female embryos.

Copyright

This image is from Medeiros LA, Proc Natl Acad Sci U S A 2011 Aug 23;108(34):14163-8. Copyright 2011 National Academy of Sciences, U.S.A. J:175228

Associated Alleles

Symbol	Name
Mirc5^tm1Jae	microRNA cluster 5, including Mir290a through Mir295; targeted mutation 1, Rudolf Jaenisch

Associated Genotypes

Allelic Composition	Genetic Background
Mirc5^tm1Jae/Mirc5^tm1Jae	involves: 129S4/SvJae * C57BL/6