Phenotype Image Detail

Image

Caption

(A) Percentage of proliferating Ki67 positive hepatocytes in Col1a1^{tm1(tetO-Tcfap2c)Hsc}/Col1a1⁺ Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺ (iTcfap2c) mice (n=5) and control (ctrl) mice (n=4) (B) Percentage of proliferating hepatocytes in cell culture, as the number of EdU positive cells. Error bars represent standard deviations. ***p<=0.0001, **p<=0.001. (C) Immunohistochemical staining of liver for Ki67 (proliferation, upper panel) and TUNEL staining positive (apoptosis, lower panel). (D) HE (middle panel) and Oil-Red O (lower panel) staining showing fat accumulation and microvisicular steatosis. (E) Tcfap2c immunohistochemistry (upper panel) and Oil-Red O staining (lower panel) of primary hepatocytes. (F) Transmission electron microscope images of mutant and ctrl liver. Hepatocytes from mutant mice are filled with lipid droplets (L); Dark and dense mitochondria in ctrl cells but bright mitochondria in mutant cells were evidence for mitochondria damage. Glycogen granules (G) were visible only in ctrl cells. Scale bars = 100 um.

Copyright

This image is from Holl D, PLoS One 2011;6(7):e22034, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:174049

Associated Alleles

Symbol	Name
Col1a1^tm1(tetO-Tcfap2c)Hsc	collagen, type I, alpha 1; targeted mutation 1, Hubert Schorle
Gt(ROSA)26Sor^tm1(rtTA*M2)Jae	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf Jaenisch

Associated Genotypes

Allelic Composition	Genetic Background
Col1a1^tm1(tetO-Tcfap2c)Hsc/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * C57BL/6