Phenotype Image Detail

Image

Caption

(A and B) Alcian blue/alizarin red-stained forelimbs of E17.5 control or Porcn^tm1.1Lcm/Y Tg(Prrx1-cre)1Cjt/0 (Prx1KO) embryos. Mutant embryos exhibit shortening of all skeletal elements and loss of distal digits. hu, humerus; ra, radius; sc, scapula; ul, ulna. (C-F) Hind-limb skeleton preparations of E18.5 control or Porcn^tm1.1Lcm/Y Tg(Msx2-cre)5Rem/0 (Msx2KO) embryos and whole-mount in situ hybridization for the AER marker Fgf8 on E11.5 hind limbs. Note the almost complete absence of autopod in E18.5 mutant and focal loss of Fgf8 expression at E11.5. (G and H) H&E-stained sections of the ventral body wall from E18.5 control or Msx2KO embryos. The brackets in H indicate an area of severe dermal thinning in mutant body wall, such that liver almost directly abuts surface ectoderm. li, liver. (I and J) Lef1 immunostaining of dorsal skin from E18.5 control or Msx2KO embryos, revealing extensive domain of hairless Lef1-devoid epidermis in mutant (arrowhead in J indicates isolated patch of Lef1⁺ basal cells). (Scale bars: G and H, 500 um; I and J, 100 um.)

Copyright

This image is from Barrott JJ, Proc Natl Acad Sci U S A 2011 Jul 18;():. Copyright 2011 National Academy of Sciences, U.S.A. J:173672

Associated Alleles

Symbol	Name
Porcn^tm1.1Lcm	porcupine O-acyltransferase; targeted mutation 1.1, Lewis Murtaugh
Tg(Msx2-cre)5Rem	transgene insertion 5, Robert E Maxson
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin

Associated Genotypes

Allelic Composition	Genetic Background
Porcn^tm1.1Lcm/Y Tg(Prrx1-cre)1Cjt/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL/J
Porcn^tm1.1Lcm/Y Tg(Msx2-cre)5Rem/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6