Phenotype Image Detail

Image

Caption

Reduction of erythrocyte PFK activity leads to hemolysis, reticulocytosis and splenomegaly. (A) PFK activity was determined in blood cell lysates from wild-type (+/+) and Pfkm^tm1Fbos/Pfkm^tm1Fbos (-/-) mice as indicated in Material and Methods. (B,C) Glucose-6-phosphate (B) and 2,3-bisphosphoglycerate (2,3-BPG) (C) concentrations were determined in blood cell perchloric extracts as indicated in Materials and Methods. (D-F) Mutants show high serum bilirubin levels (D) and reticulocyte number (E,F). New methylene blue stained blood samples were extended on slices (E) and counted (F). Arrows indicate reticulocytes. Scale bar 15um. (G,H) Splenomegaly in mutant mice. A high increase in spleen size (G) and weight (H) was observed. Scale bar 5 mm. (I,J) Hematopoietic precursors in cultured cells from spleen (I) and fermur (J) from wild-type and mutant mice. Results are mean +/- SEM of five to eight mice per group. **P<0.01 vs. wild-type.

Copyright

This image is from Garcia M, PLoS Genet 2009 Aug;5(8):e1000615, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152153

Associated Alleles

Symbol	Name
Pfkm^tm1Fbos	phosphofructokinase, muscle; targeted mutation 1, Fatima Bosch

Associated Genotypes

Allelic Composition	Genetic Background
Pfkm^tm1Fbos/Pfkm^tm1Fbos	involves: 129S6/SvEvTac * C57BL/6J