Phenotype Image Detail

Image

Caption

Increased apoptosis and defective spermatogenesis in testes by Ing2 deficiency in the absence of Trp53. (A,B) TUNEL assay in testes from 8 week old wild-type (Ing2^+/+), Ing2^tm1.1Ccha/Ing2^tm1.1Ccha (Ing2^-/-), Trp53^tm1Brd/Trp53^tm1Brd (p53^-/-) and Trp53^tm1Brd/Trp53^tm1Brd Ing2^tm1.1Ccha/Ing2^tm1.1Ccha (p53^-/- Ing2^-/-) mice. (A) The data are shown as ratios of TUNEL-positive tubules to total tubules examined (left) and as average numbers of TUNEL-positive cells per a TUNEL-positive tubule (right). At least 50 tubules were examined in each group. *P<0.001, which corresponds to P<0.05 after applying the stringent Boneferroni correction for 9 multiple comparisons. Student's test, n = 4 per group. Error bars are s.e.m. (B) Representative pictures are shown. Black arrows indicate examples of TUNEL-positive cells. Scale bars, 100 um. (C) Histological analysis of testes (top, scale bars, 100 um.) epididymis (middle, scale bars, 50 um.) and semen (bottom, scale bars, 100 um.) from 8-week old Trp53^tm1Brd/Trp53^tm1Brd and double mutant mice. Black arrows indicate degenerated large, round cells in double mutant epididymis and semen. (D) Average weight of testis from 8 week-old wild-type, Ing2^tm1.1Ccha/Ing2^tm1.1Ccha, Trp53^tm1Brd/Trp53^tm1Brd and double mutant mice. *P<0.001. Student's t test, n = 5 per group. Error bars are s.e.m.

Copyright

This image is from Saito M, PLoS One 2010;5(11):e15541, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:167311

Associated Alleles

Symbol	Name
Ing2^tm1.1Ccha	inhibitor of growth family, member 2; targeted mutation 1.1, Curtis C Harri
Trp53^tm1Brd	transformation related protein 53; targeted mutation 1, Allan Bradley

Associated Genotypes

Allelic Composition	Genetic Background
Ing2^tm1.1Ccha/Ing2^tm1.1Ccha	involves: 129 * C57BL/6J * FVB/N
Ing2^tm1.1Ccha/Ing2^tm1.1Ccha Trp53^tm1Brd/Trp53^tm1Brd	involves: 129 * 129S7/SvEvBrd * C57BL/6J * FVB/N