Phenotype Image Detail

Image

Caption

Ptk7^chz/Ptk7^chz mice exhibit reduced length to width ratio but no change in proliferation or polarity. (A) Measurements of midline axial length and width of embryos at the level of the third somite, around the time of Closure 1. (B) Comparison of length to width ratio of wild-type (+/+) (blue), Ptk7^chz/Ptk7⁺(chz/+) (green) and Ptk7^chz/Ptk7^chz (chz/chz) (red) embryos over 3-7 somite stage, demonstrating a reduced rate of increase in the length/width ratio in mutant mice, compared to wild-type and heterozygous littermates. This difference indicates a defect in convergent extension in mutant mice. (C, D) Immunostaining for phosphorylated histone H3 to examine cell proliferation in the neuroepithelium of E8.5 wild-type (C) and mutant (D) embryos. Red; phosphorylated histone H3; blue, DAPI. (E) Quantitative analysis of cell proliferation in the neuroepithelium, displayed as the percentage of cells stained for phosphorylated histone H3, revealing no significant difference in proliferation in mutant embryos. (F-I) Immunostaining of transverse sections through wild-type (F, H) and mutant (G, I) E8.5 embryos with anti-ZO-2 (F, G) and phalloidin (H, I) revealing no substantial difference in expression in mutants.

Copyright

This image is from Paudyal A, BMC Dev Biol 2010;10():87, an open-access article, licensee BioMed Central Ltd. J:163834

Associated Alleles

Symbol	Name
Ptk7^chz	PTK7 protein tyrosine kinase 7; chuzhoi

Associated Genotypes

Allelic Composition	Genetic Background
Ptk7^chz/Ptk7^chz	involves: BALB/cAnN * C3H/HeH