Phenotype Image Detail

Image

Caption

Ptk7^chz/Ptk7^chz mice exhibit a failure of closure 1 and a broadened midline. (A, B) Dorsal views of 6-somite E8.5 wild-type (+/+) (A) and Ptk7^chz/Ptk7^chz (chz/chz) (B) embryos demonstrating initiation of neural tube closure in the wild-type (arrow A) but failure to initiate neural tube closure in the mutant. (C, D) Lateral views of E9.0 wild-type (C) and mutant mice (D) embryos; arrow marks the most posterior region of closed neural tube. The wild-type embryo has completed closure in the head and only the posterior neuropore remains open in the spine, whereas the mutant embryos exhibits closure in the forebrain but remains open from the midbrain along the length of the spinal neural tube. (E, F) Transverse sections stained with hematoxylin and eosin through the caudal end of 5-somite wild-type (E) and mutant mice (F) embryos, revealing a compact ventral midline hinge point in wild-type (arrowhead, E) but a broadened ventral region and split median hinge point in mutants (arrowheads, F). (G, H) Wholemount in situ hybridization for brachyury expression in E8.5 wild-type (G) and mutant mice (H) embryos demonstrates broadened ventral midline in mutants, particularly in posterior regions (arrow, H). (I-L) Wholemount in situ hybridization for nodal expression in E8.0 (head-fold stage) (I, J) and E7.5 (K, L) wild-type (I, K) and mutant (J, L) embryos demonstrates shortened and broadened node area in mutants at headfold stage (J), while the shape of the node is not obviously altered at E7.5.

Copyright

This image is from Paudyal A, BMC Dev Biol 2010;10():87, an open-access article, licensee BioMed Central Ltd. J:163834

Associated Alleles

Symbol	Name
Ptk7^chz	PTK7 protein tyrosine kinase 7; chuzhoi

Associated Genotypes

Allelic Composition	Genetic Background
Ptk7^chz/Ptk7^chz	involves: BALB/cAnN * C3H/HeH