Phenotype Image Detail

Image

Caption

Day 42 testes were Bouin's-fixed, PAS-stained and hemtoxyin-counter-stained to visualize the glycoproteins/acrosome (pink) and nuclei (blue) and analyzed by light microscopy using a 40x objective lens. Stages are labeled with Roman numerals. (A-C) Wild-type (+/+) seminiferous tubules displayed evenly spaced round and elongated spermatids. (D) Ehd1^tm1.2Haba/Ehd1^tm1.2Haba (-/-) seminiferous tubules contained step 9, 10, and 11 elongated spermatids (arrows) and misoriented step 16 elongated spermatids near the basement membrane (circles) and (E) abnormal step 9 spermatids along with aggregates of step 16 spermatids (circles). (F-H) Mutant seminiferous tubules displayed membranous wheels or residual bodies (arrows, Rb) containing clumped spermatids near the lumen in stage VIII, (I) misaligned spermatids (circle) and clumped step 16 spermatid nuclei (arrows) in stage X, (J) abnormal step 11 spermatids in stage XI and step 16 spermatids clumping in membranous wheel in stage VIII. Scale bar = 50 um.

Copyright

This image is from Rainey MA, BMC Dev Biol 2010;10():37, an open-access article, licensee BioMed Central Ltd. J:160452

Associated Alleles

Symbol	Name
Ehd1^tm1.2Haba	EH-domain containing 1; targeted mutation 1.2, Hamid Band

Associated Genotypes

Allelic Composition	Genetic Background
Ehd1^tm1.2Haba/Ehd1^tm1.2Haba	involves: 129P2/OlaHsd * C57BL/6J * FVB/N