Phenotype Image Detail

Image

Caption

(A) Representative Hematoxylin & Eosin (H&E) stained transverse sections, showing aortic stenosis (AS, arrow) in Sos1^tm1.2Rak/Sos1⁺ (+/EK) hearts. Ao, aorta. Original magnification, x10. (B) Representative Doppler image, with measurement of ascending aortic peak velocity of an 8-month-old heterozygous mutant mouse. (C) Representative M-mode (left parasternal short axis view; top) and 2D (left parasternal long and short axes; bottom) echocardiographic images obtained from 8-month-old mice. Green bars (bottom) indicate the thickness of interventricular septum. IVS, IVS thickness; LVId, left ventricular dimension at diastole; LVIs, left ventricular dimension at systole; LVPW, left ventricular posterior wall thickness. (D-F) Representative Masson trichrome staining showing fibrosis in the hearts of a 9-month-old heterozygous mutant mouse (right) but not in a wild-type (+/+) littermate (left). (D) Original magnification, x4. (E and F) Original magnification, x10. (G) Immunofluorescence staining of collagen III (Col III) in the left ventricular wall. Original magnification, x40. (H) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay costained with the cardiomyocyte marker Troponin T, showing cardiomyocyte death in a heterozygous mutant heart; the right panel shows a higher magnification view. Arrows indicate TUNEL positive cells. Original magnification, x40 and x64. (I) Representative quantitative real-time PCR results, showing increased expression of cardiac hypertrophic markers in the heterozygous mutant hearts. Error bars indicate SD. **P < 0.01; ***P < 0.001.

Copyright

This image is from Chen PC, J Clin Invest 2010 Dec 1;120(12):4353-65 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:171873

Associated Alleles

Symbol	Name
Sos1^tm1.2Rak	SOS Ras/Rac guanine nucleotide exchange factor 1; targeted mutation 1.2, Raju Kucherlapati

Associated Genotypes

Allelic Composition	Genetic Background
Sos1^tm1.2Rak/Sos1^+	involves: 129S/Sv * C57BL/6 * FVB/N * SJL