Phenotype Image Detail

Image

Caption

(A and B) Immunofluorescence staining for BrdU (top rows) and TUNEL (bottom rows) in the (A) interventricular septum and (B) aortic valve leaflets of E15.5 wild-type (+/+), Sos1^tm1.2Rak/Sos1⁺ (+/ERK), and Sos1^tm1.2Rak/ Sos1^tm1.2Rak (EK/EK) embryos. Graphs show quantification with error bars indicating SD. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with wild-type littermates. (C) Hematoxylin & Eosin (H&E) stained sections showing aortic stenosis (AS) in 3-month-old homozygous mutant mice. (A-C) Original magnification, x4. (D) Masson trichrome staining of heart tissue, showing fibrosis in 3-month-old homozygous mutant mice. Note blue collagen staining in the left atrial (LA) epicardium (blue arrow) (left panel original magnification, x4), with a magnified view in the middle panel (original magnification, x20), as well as in right ventricular epicardium (right panel, original magnification, x10). (E) Masson trichrome staining of homozygous mutant hearts. Black arrows indicate degenerating cardiomyocytes. Blue arrows indicate adipocytes in the area surrounding (or around) the blood vessels (left panel, original magnification, x10) and within the interventricular septum (right panel, original magnification, x20).

Copyright

This image is from Chen PC, J Clin Invest 2010 Dec 1;120(12):4353-65 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:171873

Associated Alleles

Symbol	Name
Sos1^tm1.2Rak	SOS Ras/Rac guanine nucleotide exchange factor 1; targeted mutation 1.2, Raju Kucherlapati

Associated Genotypes

Allelic Composition	Genetic Background
Sos1^tm1.2Rak/Sos1^tm1.2Rak	involves: 129S/Sv * C57BL/6 * FVB/N * SJL