Phenotype Image Detail

Image

Caption

(A-C) Wild-type testes. Most tubules were normal in appearance, and stages of spermatogenesis consisted of the correct cellular associations. (A) Wild-type stage VII-VIII, consisting of step-7-8 and step-16 elongate spermatids (near lumen). (B) Wild-type stage XI, with step-11 elongating spermatids. (C) Wild-type stage XII with spermatocytes exhibiting condensed chromatin of meiosis 2 and step-12 spermatids. (D-I) Cstf2t^tm1Ccma/Cstf2t^tm1Ccma (Cstf2t^-/-) testes. (D) Mutant testis in stage IX, with normal step-9 spermatids but with abnormal retention of step-16 spermatids (arrowheads), indicating failed spermiation. Large abnormal aggregates of residual bodies are seen attached to the retained spermatids (arrows). (E) Mutant testis showing normal step-8 spermatids along with abnormal step-16 spermatids that are not aligning properly for spermiation (arrowheads). Abnormal residual bodies form near the lumen (arrows). (F) Mutant stage XII containing several abnormalities, including degenerative spermatocytes in meiosis 2 (arrows, with PAS+ granulation), binucleate step-1 spermatids (circles), and abnormal elongated spermatid heads (arrowheads). (G) Mutant stage I with normal step-1 spermatids, numerous abnormally shaped step-13 elongated spermatid heads (arrowheads) and evidence of sloughing of round spermatids (arrows). (H) Mutant stage IX, with normal step-9 spermatids but showing abnormal step-16 spermatid heads (arrowheads) being retained within the epithelium. (I) Mutant stage X, with some normal elongating spermatids (10) but also showing early formation of misshapen spermatid heads (arrowheads). Pachytene spermatocytes are also seen near the lumen, where they may be sloughed (arrow). (Scale bar: A-I, 25 um.) (J) Mutant mouse testis at lower magnification to show round spermatids (arrowheads), spermatocytes (arrows), and residual body debris (arrows) being sloughed into the lumen. (Scale bar, 50 um.) (K) Mutant mouse epididymis showing evidence of extensive sloughing of germ cells and debris by the testis (arrowheads).

Copyright

This image is from Dass B, Proc Natl Acad Sci U S A 2007 Dec 18;104(51):20374-9. Copyright 2007 National Academy of Sciences, U.S.A. J:130584

Associated Alleles

Symbol	Name
Cstf2t^tm1Ccma	cleavage stimulation factor, 3' pre-RNA subunit 2, tau; targeted mutation 1, Clinton C MacDonald

Associated Genotypes

Allelic Composition	Genetic Background
Cstf2t^tm1Ccma/Cstf2t^tm1Ccma	involves: 129S/SvEv * C57BL/6