Phenotype Image Detail

Image

Caption

Analysis of chondrocyte apoptosis and vascular invasion in skeletal elements. von Kossa staining of the tibia (A and B) and humerus (C and D) at E15.5, suggesting normal mineralization of the extracellular matrix (ECM) in Mbtps1^tm1Jdh/Mbtps1^tm1Jdh Tg(Col2a1-cre)1Bhr/0 (S1P^cko) mice. The black arrows (B and D) depict areas of abnormal mineralization. Increased chondrocyte apoptosis as detected by a TUNEL assay is seen in the humerus (F) and scapula (H) of mutant mice at E15.5. In E15.5 wild-type (WT) humorus (E) or scapula (G), hardly any apoptosis is observed; some apoptosis is seen at the chondroosseous junction in the humerus (E, white arrows). I: PECAM-1 IHC showing the presence of blood vessels in the developing bone (b) of the wild-type humerus and its absence in the cartilage (c) at E15.5. J: PECAM-1 staining is absent in the diaphysis (d) of the mutant humerus at E15.5 but is evident in the anomalous bone collar (bc). The dotted lines in panels I and J demarcate the humerus from the surrounding tissue and also outline the bone collar in the mutant. Bars: (A-D) 50 um; (E-J) 200 um.

Copyright

This image is from Patra D, J Cell Biol 2007 Nov 19;179(4):687-700, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:135374

Associated Alleles

Symbol	Name
Mbtps1^tm1Jdh	membrane-bound transcription factor peptidase, site 1; targeted mutation 1, Jay D Horton
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
Mbtps1^tm1Jdh/Mbtps1^tm1Jdh Tg(Col2a1-cre)1Bhr/0	involves: 129S6/SvEvTac * C57BL/6 * SJL