Phenotype Image Detail

Image

Caption

Ultrastructural analysis of cardiac sarcomere maturation. A: Sarcomeres assemble in both wild-type (WT) and Ttn^tm1.1Her/Ttn^tm1.1Her (KO) mice, but lateral growth is impaired from day 10 in knockout mice followed by sarcomere disassembly (h and j). At E11, nearly all sarcomeric structures are dissolved. B: Myofibril diameter was quantified for >50 fibers in duplicate with two animals per group. The lateral growth of mutant myofibrils lags behind the increase in wild-type myofibril diameter. The loss of sarcomere structure precludes the analysis of E10.5 and 11.0 sarcomeres in mutant mice. **, P < 0.01. C: Sarcomere length was not changed in wild-type versus mutant mice before disassembly at E10.5. Error bars represent SD. D: M-band and Z-disc structures at E10.0 do not differ significantly between mutants and wild-type mice, and there is no change in the A/l alignment. Slight variation in M-band alignment is present in both mutant and wild-type mice (arrowheads in a and b). Embryonic sarcomeres do not display an electron-dense M band. L, lipid; Mi, mitochondrium; N, nucleus; Z, Z disc; M, M band. Bars, 0.5 um.

Copyright

This image is from Weinert S, J Cell Biol 2006 May 22;173(4):559-70, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:111284

Associated Alleles

Symbol	Name
Ttn^tm1.1Her	titin; targeted mutation 1.1, Joachim Herz

Associated Genotypes

Allelic Composition	Genetic Background
Ttn^tm1.1Her/Ttn^tm1.1Her	involves: 129S4/SvJae * 129T2/SvEms