Phenotype Image Detail

Image

Caption

Morphological and physiological abnormalities of Syna^tm1.1Ics/Syna^tm1.1Ics embryos and extraembryonic tissues. A: Mean fetal weight of wild-type (SynA^+/+), heterozygous, and Syna^tm1.1Ics/Syna^tm1.1Ics (SynA^-/-) embryos at different embryonic ages (E). Values (milligrams) are means +/- SEM of a minimum of 7 living embryos (heart beating): 158 +/- for wild-type vs. 121 +/- 5 for mutant embryos (P = 0.0005) at E13.5; no difference between wild-type and heterozygous embryos. B-E: Vascularization of E13.5 wild-type (Left) and living mutant (Right) embryos and of their extraembryonic annexes. B: A representative homozygous mutant embryo, smaller and slightly paler than its wild-type littermate. C and D: Two opposite views of yolk sacs with the blood vessels (arrows) pale and barely visible for the mutant. E: Fetal side of placentae showing reduced vascularization in the mutant. (Original magnification: B, 12x; C and D, 15x; and E, 25x.) F: Transplacental passage of rhodamine 123 into E11.5 embryos 2 hours after i.p. injection of the fluorescent dye into the mother. (Right) Images of representative wild-type and living mutant embryos from the same litter. (Left) Quantification of the fluorescence intensity for wild-type and mutant embryos from 9 independent litters. For each litter, the mean fluorescence intensity of the wild-type embryos is set as 100%, and the relative intensity of each littermate is plotted. Two-tailed unpaired t test indicates significant difference (P < 0.0001) between wild-type and mutant embryos (mean fluorescence intensity +/- SEM: 85.15 +/- 2.2, n= 17 for mutant vs. 100 +/- 0.6, n= 16 for wild-type embryos). Only living embryos were analyzed.

Copyright

This image is from Dupressoir A, Proc Natl Acad Sci U S A 2009 Jul 21;106(29):12127-32. Copyright 2009 National Academy of Sciences, U.S.A. J:150813

Associated Alleles

Symbol	Name
Syna^tm1.1Ics	syncytin a; targeted mutation 1.1, Mouse Clinical Institute

Associated Genotypes

Allelic Composition	Genetic Background
Syna^tm1.1Ics/Syna^tm1.1Ics	involves: 129S2/SvPas * BALB/c * C57BL/6 * SJL