Phenotype Image Detail

Image

Caption

Ultrastructure showing apoptosis in basal epidermal layer and mitotically active basal-like cells in suprabasal pearls. Electron microscopy of basal layer of Itgb4^tm1Efu/Itgb4^tm1Efu newborn mouse skin, fixed, and embedded in Epon. A-B: Basal layer, separated from underlying basal lamina (double arrowheads denote separation), showing numerous examples of cells displaying highly condensed chromatin and condensed cytoplasm, giving the cells an electron dense appearance. All seven cells show some signs of apoptosis, and the first, fourth, and seventh cells in the row are in the most advanced stages. Cell in B is higher magnification of first cell in A. Note the highly condensed chromatin and signs of degeneration of mitochondria. C: Boundary of a pearl of cells within the suprabasal layer. Arrowheads denote border: cells above are within the pearl; those below are normal looking spinous cells. Note cell in prophase (big arrow). D: Higher magnification of a boundary region, showing normal looking spinous cell at left and cytoplasms of three different pearl cells to the right. Note abundance of keratin filaments (kfs) and desmosomes (de) in the spinous cell, but their paucity in the immature looking cells within the pearl. Inset shows keratin filaments and desmosome within a pearl cell, demonstrating that these cells are keratinocytes. Bar in A represents 1.9 um in A; 0.8 um in B; 3.3 um in C; and 1 um in D; 1.6 um inset to D.

Copyright

This image is from Dowling J, J Cell Biol 1996 Jul;134(2):559-72, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:77644

Associated Alleles

Symbol	Name
Itgb4^tm1Efu	integrin beta 4; targeted mutation 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Itgb4^tm1Efu/Itgb4^tm1Efu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6