Phenotype Image Detail

Image

Caption

Growth and cell cycle defects without increased apoptosis in Cert1^{Gt(RRF047)Byg}/Cert1^{Gt(RRF047)Byg} (-/-) embryos. A: Pregnant mice were injected with BrdU 1 hour before dissection, and E10.5 embryos were dissected, genotyped, and stained for BrdU uptake. Four of the areas chosen for counting the fraction of dividing cells are shown in the panels to the right. B: Mutant embryos showed a lower fraction of BrdU-positive cells, indicating a slower rate of cell division in these mutant embryos. Error bars indicate standard deviation; n = 4. C: Western blot analysis for some of the components of the cell cycle in wild-type (+/+) and mutant embryos. D: Phosphorylated Akt levels are decreased in mutant embryos. E: TUNEL staining of E10.5 wild-type and mutant embryos. Insets show boxed areas at higher magnification. There were no significant differences in the proportion of dying cells between wild-type and mutant embryos. Note that the wild-type embryos are large compared with the mutant and, therefore, were photographed twice to include the upper and lower halves of the embryo. The composites are shown in A and E.

Copyright

This image is from Wang X, J Cell Biol 2009 Jan 12;184(1):143-58, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146172

Associated Alleles

Symbol	Name
Cert1^Gt(RRF047)Byg	ceramide transporter 1; gene trap RRF047, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Cert1^Gt(RRF047)Byg/Cert1^Gt(RRF047)Byg	involves: 129P2/OlaHsd * C57BL/6