Phenotype Image Detail

Image

Caption

Histology and scanning electron micrographs of wild-type (Enam^+/+), Enam^tm1.1Jcch/Enam⁺ ((Enam^+/-) and Enam^tm1.1Jcch/Enam^tm1.1Jcch ((Enam^-/-) mice at 7 days. A, E and I, histology sections of unerupted, undelcalcified molars after von Kossa staining to show mineralization of alveolar bone, dentin, and enamel in the various genotypes, as indicated. Whereas well developed and mineralized enamel (e) and dentin (d) were present in both wild-type (A) and Enam^tm1.1Jcch/Enam⁺ (E) mice, only small, punctate foci of mineralization were detected within the enamel layer near the dentino-enamel junction, despite there being a thick accumulation of organic material in the enamel layer, presumably amelogenin and other enamel matrix constituents (I). SEM images of similar regions in each of the genotypes are shown at successively increasing magnification in each column. The forming enamel in the heterozygous mice (F-H) appears generally similar to that in wild-type (b-D) mice. The forming enamel in the Enam^tm1.1Jcch/Enam^tm1.1Jcch (J-L) mouse, despite being unmineralized, shows remarkable similarity to normal enamel matrix structure. Am, ameloblasts; Od, odontoblasts; arrowheads indicate the dentino-enamel junction.

Copyright

This image is from Hu JC, J Biol Chem 2008 Apr 18;283(16):10858-71 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:136524

Associated Alleles

Symbol	Name
Enam^tm1.1Jcch	enamelin; targeted mutation 1.1, Jan C C Hu

Associated Genotypes

Allelic Composition	Genetic Background
Enam^tm1.1Jcch/Enam^tm1.1Jcch	C57BL/6-Enam^tm1.1Jcch
Enam^tm1.1Jcch/Enam^+	C57BL/6-Enam^tm1.1Jcch