Phenotype Image Detail

Image

Caption

Global distribution of acetylcholine receptors (AChR), folds and synaptic vesicles within Snta1^tm1Scf/Snta1^tm1Scf neuromuscular junctions (NMJ). A: Sternomastoid NMJs from wild-type (alpha-Syn^+/+) and Snta1^tm1Scf/Snta1^tm1Scf (alpha-Syn^-/-) mice were labeled for AChR and imaged en face. In wild-type NMJs (a), AChR was distributed smoothly thoughout the synaptic gutter and the gutter edges appeared bright. In mutant NMJs (b-e), AChR in the gutters was distributed in streaks and clusters (b-e) and could even be absent in places (b). The gutter edges often showed little additional intensity, indicating shallow gutters. Most mutant NMJs contained lines of AChR extending beyond the gutters (b-e). Some NMJs contained areas of near normal appearance (c), whereas others were abnormal throughout (e). Bar: A, 5.0um; B, 4.8um; C, 14.7um; D, 11.4um; E, 13.1um. B: Sternomastoid NMJs from mutant mice were double- or triple-labeled as indicated. Regions of highly altered AChR distribution (a) could be largely devoid of VVA-B4 labeling (a'), suggesting absence of junctional folds. In other NMJs, virtually the entire AChR field (b) was well labeled by VVA-B4 (b'). Regions that gave strong signals for both AChR (b) and junctional folds (b'), which suggests maturity, could nevertheless be devoid of synaptophysin labeling (b"), suggesting absence of a functional nerve terminal. Bar: a and a', 12.1um; b and b', 16.1um.

Copyright

This image is from Adams ME, J Cell Biol 2000 Sep 18;150(6):1385-97, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:76455

Associated Alleles

Symbol	Name
Snta1^tm1Scf	syntrophin, acidic 1; targeted mutation 1, Stanley C Froehner

Associated Genotypes

Allelic Composition	Genetic Background
Snta1^tm1Scf/Snta1^tm1Scf	involves: 129P2/OlaHsd * C57BL/6