Gene Expression Image Detail

Reference

Figure

ID

J:113411 Corsi JM, et al., BMC Genomics. 2006;7:198

6

MGI:3805922

Image

Caption

A: RT-PCR strategy. Top: Genomic organization of the FAK (Ptk2 MGI:95481) gene (exons 12-19); alternatively spliced exons are boxed with dotted lines. The positions of the primers are indicated. PCR with the flanking forward (F2) and reverse (R2) primers amplified simultaneously all exon combinations. The selective targeting of FAK alternative transcripts was achieved with specific forward primers (F3, F4, F5, F6) coupled to the R2 primer. Each forward primer targeted transcripts with only one combination of exons 12, 13, 14 and 15; the size of the PCR product(s) distinguishes the absence or presence of exon 16 in each transcript.

Copyright

This image is from Corsi JM, BMC Genomics 2006;7():198, an open-access article, licensee BioMed Central Ltd.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
B M-F2-R2	MGI:3806079 (Ptk2)
B M-F1-R1	MGI:3806084 (Ptk2)
C M-F3-R2	MGI:3806090 (Ptk2)
C M-F5-R2	MGI:3806105 (Ptk2)
C M-F6-R2	MGI:3806106 (Ptk2)
C M-F4-R2	MGI:3806103 (Ptk2)